Endonuclease-free AAV clarification in a model system


Discover how Solventum has developed a model endonuclease-free AAV lysate clarification step.

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Learn more from two case studies with in-depth data


Case study results suggest that an efficient, endonuclease-free process may be achieved with the 3M™ Harvest RC Chromatographic Clarifier.

Designed as an efficient option to increase monoclonal antibody (mAb) yields while intensifying the upstream process, the 3M Harvest RC chromatographic clarifier is the next generation in harvest and clarification technology. The fiber non-woven functional media separates by charge, effectively capturing contaminants and impurities that are negatively charged at the harvest cell culture pH.

Applying this technology to AAV processes, the 3M Harvest RC chromatographic clarifier was studied to compare its use with and without endonuclease treatment. Results from the case study include:

  • Comparable AAV recovery (>90%) and turbidity reduction (>95%) with or without endonuclease treatment
  • DNA concentrations (>94% reduction) and characteristics in clarified lysates were comparable irrespective of endonuclease use
  • Overall predicted lower cost per batch in the endonuclease-free process
  • Similar AAV recovery and DNA reduction across the range of Harvest RC chromatographic clarifier product sizes evaluated in the case study

DNA removal case study

From discovery through each development stage, you rely on consistent performance. Study results with 3M™ Harvest RC Chromatographic Clarifier showed similarly high average AAV recovery yield at each product scale (bars) and across numerous process runs (dots) — with no endonuclease treatment of the lysate.


AAV process impact case study

At each stage of development, you need high-performance DNA reduction without endonuclease. In endonuclease-free AAV5 lysate process runs, each size of the 3M™ Harvest RC Chromatographic Clarifier achieved 94% or higher reduction in DNA — and >99% in the largest sizes evaluated.



See full data


"An investigative case study of the removal of host cell DNA from HEK detergent lysates using 3M(TM) Harvest RC Chromatographic Clarifier"
Published August 2024

"A case study for understanding impact of nuclease elimination on bioprocess cost and downstream AAV5 quality using 3M™ Harvest RC Chromatographic Clarifier"
Published August 2024


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